Cell Lines for Rare Diseases The immortalized cells have strong proliferation ability, can be passaged indefinitely, and can obtain a large number of cell resources in a shorter experimental cycle. Additionally, under the same genetic background, the stability of the experimental results can be guaranteed. A variety of cell immortalization methods have been established mainly based on the intervention of exogenous genes. Commercial cell lines including HEK293T, Hela, K562 and NIH/3T3 have been used as models for various rare diseases. Through genetic engineering, the specific genes of the cell line is mutated or overexpressed to mimic the pathogenesis of the corresponding tissues. Additionally, immortalized cells derived from the same source as the diseased tissue provide a more suitable platform for the mechanistic study of rare diseases.