How to Choose the Best 16S/18S/ITS Sequencing Method for Your Project?
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16S/18S/ITS amplicon sequencing is a prevalent method to identify taxonomic composition and complex microbial communities. The commonly used sequencing platforms include NGS platforms like Illumina MiSeqand and long-read platforms like Pacific Biosciences (PacBio) systems. Various PCR primers have been developed for this method. 16S/18S/ITS sequencing can be further subdivided into short read, absolute quantitative, and full-length 16S/18S/ITS sequencing.