Endotoxin is a kind of lipopolysaccharide that comes from the outer membrane of Gram-negative bacteria, which is released after the lysis of the bacteria. Endotoxin can elicit strong immune responses in animals and can significantly impact both in vitro and in vivo experiments, causing non-specific cellular responses and false readings in cell-based assays. Thus, it is important to limit the amount of endotoxin contaminants in injectable protein and antibody products.
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